SOFTWARE FOR IMAGE SEGMENTATION AND ANALYSIS
IN PATHOLOGY (ISAP).
TROUBLESHOOTING MENU.
Copyright © 1994 G. William Moore, Geoffrey W. Moore,
Jules J. Berman, Lawrence A. Brown.
http://www.medparse.com/isaptrub.htm
U. S. Government Work, presented at the 1994 meeting
of the American Society of Clinical Pathology, Washington, DC.
Moore GW, Berman JJ, Moore GW, Brown LA.
Software for Image Segmentation and Analysis in Pathology (ISAP)
Am J Clin Pathol 102:538-539, 1994.
1. ISAP TROUBLESHOOTING MENU.
Four menus will always be available to the user:
FILE, DATABASE, HELP, and QUIT. You can always OPEN
an existing IMAGE in uncompressed .BMP format under
the FILE menu, and you can always CONVERT an existing
IMAGE in the .TGA format under the FILE menu.
You can always display an existing DATABASE,
you can always get HELP, and you can always QUIT.
The IMAGE and EDGE options require that a .BMP
image should already be OPENed. The ANALYSIS and
VIEW ACTIVE DATABASE options require that an
IMAGE/BLOCK SELECT should already be performed
on the OPEN image.
All files must reside in the same directory as the
program file. Files may include: 8-bit uncompressed
black-and-white MS-bitmap files (name ending in .BMP);
8-bit uncompressed black-and-white Targa files (name
ending in .TGA); database files (name ending in .DBS).
BLOCK SELECT. In order to analyze a desired
particle, you must locate the particle with a
circumscribed rectangle about the particle.
Then place the pointer at the upper-left corner
of the particle. Then click the mouse (left) button,
and drag the mouse to the lower-right corner of the
particle. Finally, release the mouse button. Then
the software will identify the edge of the particle,
and draw an edge around the particle. Select
ANALYSIS or DATABASE to view data for the
particle just selected.
PERIMETER ERROR. The edge-detection method
must find a continuous perimeter around the particle
within the select-block. This error may occur
because the particle touches the edge of the
select-block, because the particle has a very
irregular edge, because the particle is fragmented,
or because the THRESHOLD is misplaced. You may
resize the BLOCK SELECT (IMAGE menu) and try again.
If the image is dark, then move the THRESHOLD
to a lower value. If the image is light, then
move the THRESHOLD to a higher value.
Last Updated: January 23, 2003, by G. William Moore, MD, PhD.