# ANALYSIS menu: There are two submenus: HISTOGRAM, VALUES. HISTOGRAM submenu. If there is an OPEN IMAGE (FILE menu) on the screen, and a particle has been BLOCK SELECTed (IMAGE menu), then the HISTOGRAM submenu of the ANALYSIS menu displays a bar graph of the gray values in the particle. The RED BARs represent the distribution of gray values. The BLUE BAR is the arithmetic mean for the distribution of gray values. The YELLOW BAR is a DIVIDER for a bimodal distribution, calculated by dividing the histogram at all possible points along the x-axis, and selecting the minimum pooled Student t standard deviation. VALUES submenu. If there is an OPEN IMAGE (FILE menu) on the screen, and a particle has been BLOCK SELECTed (IMAGE menu), then the VALUES submenu of the ANALYSIS menu displays particle area (AREA), sum of gray values in the particle (GRAYSUM), sum of optical densities in the particle (DENSESUM), average optical density (DENSEAVG), standard deviation of optical density (DENSESTDV), least standard error divider (DIVIDER), average diameter of the particle (AVGDIAM), greatest diameter (MAXDIAM), least diameter (MINDIAM), elliptical eccentricity (ECCENT), center-of-gravity x-axis (XCENTER) and y-axis (YCENTER), particle contour (CONTOUR), fractal dimension (FRACTAL), and perimeter (PERIMETER). The AREA is the number of pixels INSIDE the particle. GRAYSUM is the sum of GRAY_VALUEs inside the particle. From each GRAY_VALUE, the OPTICAL_DENSITY for that point is calculated by the formula: OPTICAL_DENSITY=LOG10(255/(255-GRAY_VALUE)), with OPTICAL_DENSITY equal zero if GRAY_VALUE equals 255. The DENSESUM is the sum of OPTICAL_DENSITY values inside the particle. DENSEAVG equals DENSESUM/AREA, and is designated with a BLUE BAR on the HISTOGRAM. DENSESTDV is the standard deviation of OPTICAL_DENSITY values inside the particle. If the x-axis of the HISTOGRAM is divided at all points, and a Student t pooled standard error is calculated for the left and right histograms at every point. The minimum pooled standard error at the DIVIDER, and designated with a YELLOW BAR on the HISTOGRAM. The MAXDIAM for the particle is the (Euclidean) distance between the two most distant edge-points. If the particle is assumed to be an ellipse, then: AREA = (pi/4) * MAXDIAM * MINDIAM, and ECCENT = MAXDIAM/MINDIAM. If the particle is assumed to be a circle, then: AREA = (pi/4) * AVGDIAM * AVGDIAM. PERIMETER is the sum of edge-points of the particle. The particle CONTOUR equals PERIMETER/(2*sqrt(pi*AREA)). For a perfect circle, the CONTOUR assumes a minimum value of one. The `fractal dimension', s, of a particle is a quantitative measure of complexity along the particle edge. s = 1 + lim(d->0) log2((PERIMETER at 2*d)/(PERIMETER at d)), for GRIDSIZE=d, as GRIDSIZE approaches 0. ISAP(c) calculates s for GRIDSIZE=1 pixel. #